1.     What are the definitions and meanings of two parameters,  Km and vmax, in Michaelis-Menten equation? State in chemical & biological engineering aspects. [3pt]

- Km

(k2 + k3) / k1

vmax/2

substrate affinity with enzyme

-vmax

k2[E]

maximum reaction rate

reaction rate when all enzymes are saturated with substrates

2. There were many chemical species involved in this experiment ;Glucose, glucoseoxidase, gluconolactone, peroxide, peroxidase, o-dianisidine. State which species(except for gluconolactone and peroxidase) are reductants or oxidants. [2pt]

reductant : glucose, o-dianisidine(e-donor)

oxidant : glucoseoxidase(GOD), peroxide

3. (attachment)

Regards,

TA.